KLF4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	KLF4 antibody can be used for the detection of KLF4 by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 µg/mL. <br><br> Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLF4 antibody was raised against a 20 amino acid peptide near the carboxy terminus of human KLF4.
Formulation	PBS containing 0.02% sodium azide.
Purification	Affinity chromatography purified via peptide column
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Kruppel-like factor 4 (gut)
Database Link	AAH30811 Entrez Gene 9314 Human UniProt ID O43474
Background	KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.
Synonyms	EZF; GKLF
Reference Data

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