NANOG Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ICC/IF, IHC, WB
Recommend Dilution	WB: 1-2 µg/mL ICC/IF: 20 µg/mL IHC: 1-5 µg/mL. <br> Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	NANOG antibody was raised against a 19 amino acid peptide near the center of human NANOG.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	Affinity chromatography purified via peptide column
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Nanog homeobox
Database Link	NP_079141 Entrez Gene 71950 MouseEntrez Gene 414065 RatEntrez Gene 79923 Human UniProt ID Q9H9S0
Background	Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Synonyms	homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Reference Data
Protein Families	Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell - Pluripotency

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