XBP1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ICC, IF, IHC, WB
Recommend Dilution	WB: 0.5 - 2 ug/mL ICC: 10 ug/mL WB: 0.25-2 µg/mL; IF: 20 µg/mL; ICC: 10 µg/mL; IHC: 2-5 µg/mL.<br>Antibody validated: Western Blot in human samples; Immunofluorescence in human and rat samples; Immunocytochemistry in human samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	XBP-1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	Affinity chromatography purified via peptide column
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	X-box binding protein 1
Database Link	NP_001073007 Entrez Gene 22433 MouseEntrez Gene 7494 Human UniProt ID P17861
Background	X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94 (3,4).
Synonyms	TREB-5; TREB5; XBP-1; XBP2
Reference Data
Protein Families	Transcription Factors

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