ICAD (DFFA) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: 0.5 - 1 ug/mL, ICC: 5 ug/mL
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	Antibody is DEAE purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	DNA fragmentation factor subunit alpha
Database Link	NP_004392 Entrez Gene 1676 Human UniProt ID O00273
Background	Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
Synonyms	DFF-45; DFF1; ICAD
Reference Data
Protein Pathways	Apoptosis

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