SIAH1 Goat Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: 0.1-0.3µg/ml, 1 h at RT. Approx. 37kDa band observed in Human Liver lysates and approx. 30+37kDa bands in Rat Liver lysates (calculated MW of 34.6kDa according to Human NP_001006611.1 and 31.1kDa according to Rat NP_543181.2 and 38.2kDa according to Rat XP_038953364.1). Immunofluorescence: 10µg/ml. Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells.
Reactivity	Human, Rat, Mouse, Dog, Cow
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3; NP_001006611.1.
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	34.4 kDa
Gene Name	siah E3 ubiquitin protein ligase 1
Database Link	NP_001006611 Entrez Gene 140941 RatEntrez Gene 100856496 DogEntrez Gene 6477 Human UniProt ID Q8IUQ4
Background	This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]
Synonyms	SIAH1A
Reference Data
Protein Families	Druggable Genome
Protein Pathways	p53 signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway

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