TRIAD3 (RNF216) (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot (0.5 - 1.0 µg/ml, detects a band of approximately 95 kDa in mouse heart cell lysates). Immunohistochemistry on paraffin sections.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A 15 amino acid peptide located near mouse TRIAD3A amino-terminus
Specificity	This antibody recognises an epitope within the N-terminal region (NT) of TRIAD3A.
Formulation	Phosphate buffered saline, 0.02 % Sodium Azide State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Name	ring finger protein 216
Database Link	UniProt ID Q9NWF9
Background	TRIAD3A is a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response. Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.
Synonyms	RING finger protein 216, TRIAD3, UBCE7IP1, ZIN
Reference Data

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