Caspase 4 (CASP4) (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1.0 µg/ml. Immunohistochemistry on paraffin sections: 2.0 µg/ml. This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Spleen tissue.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A 16 amino acid peptide from Human Caspase-4 amino terminus
Specificity	This antibody recognises an epitope within the N-terminal region (NT) of Caspase-4, otherwise known as ICH-2. This antibody detects a band of approximately 34 kDa in Ramos cell lysates (predicted precursor MWT 31 kDa).
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	caspase 4
Database Link	Entrez Gene 837 Human UniProt ID P49662
Background	Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimers disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis.
Synonyms	CASP4, ICH2, ICE(rel)-II, Protease ICH-2, Protease TX
Reference Data

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