PIN1 Mouse Monoclonal Antibody [Clone ID: 3G8]

Specifications

Product Data
Clone Name	3G8
Applications	ELISA, FC, WB
Recommend Dilution	ELISA. Western blot: 1/500-1/1,000 (Recommended starting dilution is 1/500). Flow Cytometry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human Pin1 (1-163 aa) purified from E. coli
Specificity	This antibody is specific for Human Pin1. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Database Link	Entrez Gene 5300 Human UniProt ID Q13526
Background	Human Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
Synonyms	PIN-1
Reference Data

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