CD45 / LCA Mouse Monoclonal Antibody [Clone ID: K252-1E4]

Specifications

Product Data
Clone Name	K252-1E4
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine Peripheral Blood lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
Specificity	This antibody recognizes all Porcine leucocytes. This K252.1E4 antibody recognizes an epitope common to all porcine CD45 isoforms (Schnitzlein et al. 1998). K252.1E4 immunoprecipitates three polypeptides of 226, 210 and 190kDa from preparations of porcine peripheral blood mononuclear cells and shows a broad reactivity pattern with both lymphoid and myeloid cells (Zuckermann et al. 1994).
Formulation	PBS Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q6SZ85
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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