CD44 Mouse Monoclonal Antibody [Clone ID: MEM-85]

Specifications

Product Data
Clone Name	MEM-85
Applications	ELISA, FC, IHC, IP, WB
Recommend Dilution	ELISA. Flow Cytometry: 1 µg/ml. Immunoprecipitation. Western Blot: 2 µg/ml, 60 min on vertical incubator Positive Control: Kg-1a human leukemia cell lysate. Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Immunohistochemistry on Paraffin Sections: 5 µg/ml. Formalin-Fixed Praffin-Ebedded Hman tissues requires HIER antigen retrieval with sodium citrate at pH 6.0.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Leucocytes of a patient suffering from LGL Type Leukaemia
Specificity	The antibody MEM-85 reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
Formulation	PBS, pH~7.4 with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD44 molecule (Indian blood group)
Database Link	Entrez Gene 960 Human UniProt ID P16070
Background	CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Synonyms	LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1
Reference Data

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