IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: MEM-140]

Specifications

Product Data
Clone Name	MEM-140
Applications	FC, IP
Recommend Dilution	Flow Cytometry: 1:200 (starting point).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	PHA-activated peripheral blood leucocytes
Specificity	This antibody reacts with CD25 (Interleukin-2 receptor a chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Label: Biotin State: Liquid purified Ig fraction Label: Conjugated with -LC-NHS under optimum conditions. The reagent is free of unconjugated biotin
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
Gene Name	interleukin 2 receptor subunit alpha
Database Link	Entrez Gene 3559 Human UniProt ID P01589
Background	CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
Synonyms	Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.