SOCS3 Mouse Monoclonal Antibody [Clone ID: SO1]

Specifications

Product Data
Clone Name	SO1
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot (Reducing conditions): Positive Control: Use HeLa human cervix carcinoma cell line. Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including protease inhibitor cocktail). Incubate 60 min on ice, centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. This Clone SO1 is reported to work on Paraffin Sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length SOCS3 protein.
Specificity	This antibody SO1 reacts with SOCS3, an intracellular Cytokine Signaling Inhibitor.
Formulation	PBS with 15 mM Sodium Azide as preservative, approx. pH 7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	suppressor of cytokine signaling 3
Database Link	Entrez Gene 9021 Human UniProt ID O14543
Background	SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.
Synonyms	SOCS-3, CIS-3, SSI3, SSI-3
Reference Data

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