Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: VI-RE/1]

Specifications

Product Data
Clone Name	VI-RE/1
Applications	FC
Recommend Dilution	Flow Cytometry (starting point 10 μg/ml).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed full-length Human Vimentin
Specificity	The antibody VI-RE/1 reacts with vimentin.
Formulation	Phosphate buffered saline (PBS) containing 15 mM Sodium Azide and 0.2% (w/v) high-grade protease free BSA as a stabilizing agent. Label: PE State: Liquid purified Ig fraction Label: Conjugated with R-Phycoerythrin under optimum conditions. The conjugate is purified by size-exclusion chromatography
Concentration	lot specific
Purification	Size-Exclusion Chromatography.
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin. Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with glial filament acidic protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Synonyms	VIM
Reference Data

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