SYK (5-360) Mouse Monoclonal Antibody [Clone ID: SYK-01]

Specifications

Product Data
Clone Name	SYK-01
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections (5 μg/ml). Positive Tissue: Tonsil B cells. Immunocytochemistry. Immunoprecipitation. Western blot (1-2 µg/ml, 60 min). Positive Control: RBL rat basophilic leukemia cell line, A-431 human epidermoid carcinoma cell line, RAMOS lymphoma cell line, U-937 human histiocytic lymphoma cell line, JURKAT human peripheral blood T cell leukemia cell line. Negative Control: HeLa human cervix carcinoma cell line. Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12% separating gel).
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant fragment (aa 5-360) of Human Syk
Specificity	The monoclonal SYK-01 antibody reacts with Protein Tyrosine Kinase p72Syk (Syk; Syk family tyrosine-specific phospho-transferase), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FceRI).
Formulation	Phosphate buffered saline (PBS), pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	spleen tyrosine kinase
Database Link	Entrez Gene 6850 Human UniProt ID P43405
Background	Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FceRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Synonyms	Tyrosine-protein kinase SYK, Spleen tyrosine kinase
Reference Data

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