alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: AFP-01]

Specifications

Product Data
Clone Name	AFP-01
Applications	ELISA, IF, IP, WB
Recommend Dilution	Immunoprecipitation: Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. ELISA: The antibody has been tested as the detection antibody in a Sandwich ELISA for analysis of human alpha-Fetoprotein in combination with clone AFP-11 (cat. no. SM3090P). Western blot: 1-2 μg/ml; non-reducing conditions. Positive Material: Hep G2 human hepatocellular carcinoma cell line.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified alpha-Fetoprotein
Specificity	The antibody reacts with 70 kDa alpha-Fetoprotein (AFP).
Formulation	Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Precipitation Methods
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	alpha fetoprotein
Database Link	Entrez Gene 174 Human UniProt ID P02771
Background	AFP is an oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Synonyms	Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP
Reference Data

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