PAG (PAG1) Mouse Monoclonal Antibody [Clone ID: MEM-255]

Specifications

Product Data
Clone Name	MEM-255
Applications	FC, IHC, WB
Recommend Dilution	Flow Cytometry: 2 mg/ml (intracellular staining, permeabilization required). Western blot: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG.
Specificity	The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
Formulation	PBS, pH~7.4 containing 15 mM Sodium Azide State: Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
Concentration	lot specific
Purification	Protein-A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	phosphoprotein membrane anchor with glycosphingolipid microdomains 1
Database Link	Entrez Gene 55824 Human UniProt ID Q9NWQ8
Background	PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Synonyms	PAG, CBP
Reference Data

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