CD8A Mouse Monoclonal Antibody [Clone ID: MEM-87]

Specifications

Product Data
Clone Name	MEM-87
Applications	FC, IP
Recommend Dilution	Immunoprecipitation (Excellent for immunoisolation of CD8 + T cells). Flow Cytometry: 2 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Leucocytes of a patient suffering from LGL Type Leukaemia
Specificity	The antibody recognizes CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	CD8a molecule
Database Link	Entrez Gene 925 Human UniProt ID P01732
Background	The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as ab heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8b level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 a-b but not a-a dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. CD8 is a disulfide-linked dimer and exists as a CD8a homodimer or CD8a/b heterodimer (each monomer approx. 32-34 kDa).
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways	Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway

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