CD34 Class III Mouse Monoclonal Antibody [Clone ID: 4H11]

Specifications

Product Data
Clone Name	4H11
Applications	FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: 2 µg/ml. Western blot (non-reducing conditions): 2 µg/ml. Positive control: Kg-1a human leukemia cell lysate. Negative control: JURKAT human leukemia T-cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Immunohistochemistry on Paraffin Sections: 10 μg/ml. Positive tissue: placenta endothelium.  Immunocytochemistry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
Specificity	The antibody reacts with Class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Formulation	PBS pH 7.4 with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
Concentration	lot specific
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD34 molecule
Database Link	Entrez Gene 947 Human UniProt ID P28906
Background	CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Synonyms	Hematopoietic progenitor cell marker
Reference Data

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