CD3E Mouse Monoclonal Antibody [Clone ID: MEM-57]

Specifications

Product Data
Clone Name	MEM-57
Applications	FC, FN, IP
Recommend Dilution	Flow Cytometry: 2-5 µg/ml Positive Control: Peripheral Blood Lymphocytes, Jurkat T cell line. Immunoprecipitation: The antibody immuno-precipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7). Functional Application: MEM-57 activates T lymphocytes in vitro. It has a strong Mitogenic effect, it reacts strongly with Gamma/Delta Lymphocytes.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymocytes and T lymphocytes
Specificity	The antibody reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
Formulation	Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD3e molecule
Database Link	Entrez Gene 916 Human UniProt ID P07766
Background	CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms	T3/Leu-4
Reference Data

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