Integrin beta 1 (ITGB1) Mouse Monoclonal Antibody [Clone ID: MEM-101A]

Specifications

Product Data
Clone Name	MEM-101A
Applications	FC
Recommend Dilution	Flow Cytometry (Indirect Immunoflourescence): Use 1/1000 dilution as starting point.
Reactivity	Canine, Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Raji Burkitt's lymphoma cell line
Specificity	The antibody clone MEM-101A reacts with CD29 (integrin b1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin a subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).
Formulation	Phosphate buffered saline (PBS), pH~7.4 Label: Biotin State: Liquid purified Ig fraction Preservative: 15 mM Sodium Azide Label: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	integrin subunit beta 1
Database Link	Entrez Gene 3688 Human UniProt ID P05556
Background	CD29 (b1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 dibberent a chains (a1-a6, CD49a-f) determining the binding properties of b1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most b1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of b1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a b1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1b.
Synonyms	Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.