CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

Specifications

Product Data
Clone Name	MEM-158
Applications	FC
Recommend Dilution	Suitable for Flow cytometry ( Use 20 µl to label 10e6 cells or 100 µl whole blood).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human granulocytes
Specificity	The antibody reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
Formulation	Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent Label: FITC State: Liquid purified Ig fraction Label: Conjugated with Fluorescein isothiocyanate under optimum conditions. The reagent is free of unconjugated
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 4-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
Background	CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
Synonyms	Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1
Reference Data

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