Ptprc (CD45RA) Mouse Monoclonal Antibody [Clone ID: OX-33]

Specifications

Product Data
Clone Name	OX-33
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells in 100µl. this antibody does not always give a clear separation between positives and negatives in some cell preparations; if users find this to be the case we recommend the use of R. Phycoerythrin conjugated reagent SM279R which generally exhibits a peak of higher mean fluorescence.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Rat spleen L-CA. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Specificity	This antibody is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes.
Formulation	PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 24699 Rat UniProt ID P04157
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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