Il2ra Mouse Monoclonal Antibody [Clone ID: OX-39]

Specifications

Product Data
Clone Name	OX-39
Applications	FC
Recommend Dilution	Flow Cytometry: use 10 µl of neat antibody to label 10e6 cells in 100 µl.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Con A stimulated Rat T cells.
Specificity	Recognizes the alpha chain of rat CD25, otherwise know as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (1).
Formulation	PBS pH 7.4 with 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: PE State: Lyophilised Label: R. Phycoerythrin
Reconstitution Method	Reconstitute with 1 ml distilled water.
Purification	Protein G purified immunoglobulin fraction
Conjugation	PE
Storage Condition	Store at 2-8°C. DO NOT FREEZE. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	interleukin 2 receptor subunit alpha
Database Link	Entrez Gene 25704 Rat UniProt ID P26897
Background	The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.
Synonyms	Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.