Cd4 (Domain 2) Mouse Monoclonal Antibody [Clone ID: OX-35]

Specifications

Product Data
Clone Name	OX-35
Applications	FC, IHC, IP
Recommend Dilution	Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	MLR generated rat T helper lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises the CD4 cell surface antigen, a 55kD glycoprotein expressed by helper T cells and weakly by monocytes. This antibody recognises a different epitope on the CD4 molecule than the W3/25 monoclonal.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Cd4 molecule
Database Link	Entrez Gene 24932 Rat UniProt ID P05540
Background	CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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