Fas Ligand (FASLG) Mouse Monoclonal Antibody [Clone ID: 1OF2]

Specifications

Product Data
Clone Name	1OF2
Applications	ELISA, FC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 1x10e6 cells in 100 µl. ELISA. Immunoprecipitation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Specificity	This antibody recognises the CD178 cell surface antigen. This antibody may be used in combination with clone 14C2 (MCA2409) in ELISA assays. Clone 10F2 is reported to block the activity of Fas ligand. We recommend the use of SM2268LE for such studies.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Fas ligand
Database Link	Entrez Gene 356 Human UniProt ID P48023
Background	CD178 is a 40kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.
Synonyms	FASLG, APT1LG1, FASL, TNFSF6, CD95L protein, APTL
Reference Data

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