Fc epsilon RI (FCER1A) Mouse Monoclonal Antibody [Clone ID: 9E1]

Specifications

Product Data
Clone Name	9E1
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA. Western Blot. Immunoprecipitation. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Paraffin Sections. Heat induced antigen retrieval in pH 6.0 citrate buffer.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CCR7 Rat basophilic leukaemia (RBL)-2H3 transfectants. Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Specificity	This antibody recognizes the Human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Fc fragment of IgE receptor Ia
Database Link	Entrez Gene 2205 Human UniProt ID P12319
Background	Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.
Synonyms	IgE Fc receptor subunit alpha, Fc-epsilon RI-alpha, FCE1A, FcERI
Reference Data

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