EMR2 (ADGRE2) Mouse Monoclonal Antibody [Clone ID: 2A1]

Specifications

Product Data
Clone Name	2A1
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 10e6 cells in 100 µl. Immunoprecipitation. Western Blot. Immunohistochemistry on Frozen Sections: 1/50-1/200 dilution.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognizes EMR2, a member of the epidermal growth factor-seven transmembrane (EGFTM7) family of proteins, which is closely related to CD97. Clone 2A1 specifically recognises the stalk region of EMR2.
Formulation	PBS containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	adhesion G protein-coupled receptor E2
Database Link	Entrez Gene 30817 Human UniProt ID Q9UHX3
Background	EMR2 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2.
Reference Data

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