Itgb1 Hamster Monoclonal Antibody [Clone ID: HM beta 1-1]

Specifications

Product Data
Clone Name	HM beta 1-1
Applications	FC
Recommend Dilution	Flow Cytometry (Neat-1/10): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Reactivity	Mouse, Rat
Host	Hamster
Clonality	Monoclonal
Immunogen	Purified mouse VLA-4 antigen. Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
Specificity	This antibody recognises the integrin beta 1 subunit (CD29). Clone HM beta 1.1 is reported to inhibit beta 1 integrin mediated adhesion. We recommend the use of SM2210LE for this purpose.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	integrin beta 1 (fibronectin receptor beta)
Database Link	Entrez Gene 16412 Mouse UniProt ID P09055
Background	CD29 is a 110KD cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.
Synonyms	Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12
Reference Data

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