CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: F10-89-4]

Specifications

Product Data
Clone Name	F10-89-4
Applications	FC, IHC, IP
Recommend Dilution	Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/100 - 1/200; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; 1mM EDTA pH8.0 is recommended for this purpose. Immunoprecipitation. Flow Cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human T lymphocytes
Specificity	This antibody recognises the CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes
Formulation	Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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