CD163 Mouse Monoclonal Antibody [Clone ID: EDHu-1]

Specifications

Product Data
Clone Name	EDHu-1
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Western Blot. Immunoassay. Flow Cytometry. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections.
Reactivity	Human, Monkey, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Leucocytes harvested from pleural cavity of patients with idiopathic spontaneous pneumothorax.
Specificity	This antibody recognises the CD163 cell surface antigen. Clone EDHu-1 is reported to inhibit the binding of haptoglobin:haemoglobin to CD163 (Madsen et al. 2004).
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction from tissue culture supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD163 molecule
Database Link	Entrez Gene 9332 Human UniProt ID Q86VB7
Background	CD163 is a 130-140kD glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.
Synonyms	M130, Hemoglobin scavenger receptor, Macrophage marker
Reference Data

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