CD16/CD32 Rat Monoclonal Antibody [Clone ID: FCR 4G8]

Specifications

Product Data
Clone Name	FCR 4G8
Applications	FC
Recommend Dilution	Flow Cytometry: 1/25 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	PU5 1.8 IOE7 Balb/c mouse cell line. Cells from immunised rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Specificity	This antibody recognises an epitope expressed by the low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32).
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Afinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.
Synonyms	IgG Fc receptor III-2, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcRIIIa, Fc-gamma RIII, FcRIII, CD16a, FcR mouse Seroblock, FCGR3A, FCG3, IGFR3, Fc gamma receptor IIB, Fc-gamma-RIIB, FcRII, Ly-17, Fcgr2
Reference Data

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