Junctional Adhesion Molecule 1 (F11R) Mouse Monoclonal Antibody [Clone ID: BV16]

Specifications

Product Data
Clone Name	BV16
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunohistochemistry on frozen sections: The typical starting working dilution is 1/50. Flow cytometry: The typical starting working dilution is 1/50. Immunofluorescence. Immunoprecipitation. Western blot: The typical starting working dilution is 1/50.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein consisting of the extracellular domain of human JAM and the Fc portion of human IgGs
Specificity	The monoclonal antibody BV16 recognizes the human junction adhesion molecule (JAM)-A.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Potein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	F11 receptor
Database Link	Entrez Gene 50848 Human UniProt ID Q9Y624
Background	Together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization and their molecular weight is about 30-40 kDa. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the beta2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role for JAM-A in the initiation of atherosclerosis since JAM-A is upregulated on early atherosclerotic endothelium. The adhesion of activated platelets on the activated endothelium is mediated by homophilic interactions of JAM-A.
Synonyms	JAM-A, Platelet F11 receptor, F11R, JCAM, PAM1
Reference Data

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