Ctla4 Mouse Monoclonal Antibody [Clone ID: WKH203]

Specifications

Product Data
Clone Name	WKH203
Applications	ELISA, FC, IHC, WB
Recommend Dilution	Flow cytometry: 1:10 - 1:20; use 10 µl to label 10e6 cells in 100 µl; membrane permeabilisation is required for this application. ELISA. Western blot.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified rCTLA-4hIg fusion protein. Spleen cells from immunised Balb/c were fused with cells of the X63-Ag8-653 myeloma cell line.
Specificity	This antibody recognises CD152, also known as cytotoxic-T-lymphocyte antigen-4 (CTLA-4).
Formulation	PBS containing 0.09 % Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cytotoxic T-lymphocyte-associated protein 4
Database Link	Entrez Gene 63835 Rat UniProt ID Q62859
Background	CD152 is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes. Studies suggest that CD152 is also expressed by regulatory T-lymphocytes.
Synonyms	CTLA-4
Reference Data

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