Mannose Receptor (MRC1) Mouse Monoclonal Antibody [Clone ID: 15-2]

Specifications

Product Data
Clone Name	15-2
Applications	FC, FN, IHC, WB
Recommend Dilution	Western blot (1,2): A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa (Ref.1): The typical starting working dilution is 1:10. Flow cytometry (2): Antibody 15-2 stains the extracellular domain of MR: The typical starting working dilution is 1:10. Functional assays (1): Functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor. (Ref.1). Immunohistochemistry on frozen sections (1,2,3): Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum (Ref.3). The typical starting working dilution is 1:10. Positive control: Macrophages. Negative control: Lymphocytes and Monocytes. Does not work in Immunohistochemistry on paraffin sections (2).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human mannose receptor from human placental tissue
Specificity	The monoclonal antibody 15-2 recognizes the mannose receptor (MR), also known as CD206, a member of the vertebrate C-type lectin family. The monoclonal antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	mannose receptor, C type 1
Database Link	Entrez Gene 4360 Human UniProt ID P22897
Background	The mannose receptor, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 175 kDa single-pass type I transmembrane receptor consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR binds high-mannose structures on a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR’s are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex.
Synonyms	Macrophage mannose receptor, CLEC13D, CLEC13DL, MRC1L1
Reference Data

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