Aquaporin 1 (AQP1) (249-269) Mouse Monoclonal Antibody [Clone ID: 1/A5F6]

Specifications

Product Data
Clone Name	1/A5F6
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/1000-1/20000. Western Blot: 1/1000-1/5000. Immunoprecipitation: 1/1000-1/10000. Immunohistochemistry on Frozen and Paraffin Embedded Sections: 1/500-1/1000. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control Tissue: Kidney.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to amino acids 249-269 of Rat Aquaporin 1. Spleen cells from immunised Balb/c mice were fused with the cells of the Sp2/0 Ag14 mouse myeloma cell line.
Specificity	This antibody recognises an epitope within the cytoplasmic domain of the water-specific channel Aquaporin 1.
Formulation	State: Purified State: Liquid purified IgG fraction containing 0.09% Sodium Azide as preservative and 0.1% BSA as stabilizer.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	aquaporin 1 (Colton blood group)
Database Link	Entrez Gene 358 Human UniProt ID P29972
Background	Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Synonyms	AQP-1, CHIP28, Aquaporin-CHIP
Reference Data

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