CD21 (CR2) Mouse Monoclonal Antibody [Clone ID: LB21]

Specifications

Product Data
Clone Name	LB21
Applications	FC, IF, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells or 100 µl human whole blood. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/10-1/100. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections. Recommended Positive Control: Lymph node tissue.
Reactivity	Bovine, Feline, Goat, Human, Mink, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Human IM9 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Specificity	This antibody is a 14kD cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 3d receptor 2
Database Link	Entrez Gene 1380 Human UniProt ID P20023
Background	CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.
Synonyms	Complement receptor type 2, CR2, C3DR, C3d receptor, EBV Receptor, Dendritic Cell Marker
Reference Data

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