CD68 Mouse Monoclonal Antibody [Clone ID: 514H12]

Specifications

Product Data
Clone Name	514H12
Applications	IF, IHC
Recommend Dilution	Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/40-1/80. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH 8.0 is recommended for this purpose. The epitope recognised by clone 514H12 is sensitive to peroxide. It is therefore recommended that any peroxidase-blocking steps be performed after incubation with this antibody. Recommended Positive Control Tissue: Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein corresponding to external domain of human CD68. Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line.
Specificity	This antibody recognizes the CD68 cell surface antigen, a 110kD glycoprotein primarily expressed by macrophages and monocytes.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD68 molecule
Database Link	Entrez Gene 968 Human UniProt ID P34810
Background	The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. CD68 is an intracellular glycoprotein expressed on macrophages and monocytes. CD68 expression has been demonstrated in T cells, NK cells, liver and renal tubules.
Synonyms	Gp110, Macrosialin, Macrophage marker
Reference Data

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