6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: AD1.1.10]

CAT#: SM1693PX

6xHistidine Epitope Tag (HHHHHH) mouse monoclonal antibody, clone AD1.1.10, Purified



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CNY 21,525.00


货期*
4周

规格
    • 2 mg

Cited in 1 publication.

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Specifications

Product Data
Clone Name AD1.1.10
Applications ELISA, IHC, IP, WB
Recommend Dilution ELISA.
Immunoprecipitation.
Immunhistology on Frozen Sections.
Western blot
(1/500-1/1000): This antibody is routinely tested on Histidine tagged recombinant protein.
Host Mouse
Clonality Monoclonal
Immunogen PAX6 transcription factor linked to Histidine tag.
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. We recommend the use of SM1693P for this application.
Formulation PBS, pH 7.4
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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