Cd68 Rat Monoclonal Antibody [Clone ID: FA-11]

Specifications

Product Data
Clone Name	FA-11
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of Neat-1/10 dilueted antibody to label 106 cells in 100 µl. Membrane permeabilisation is required for this application. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Purified Concanavalin A acceptor glycoprotein from P815 cell line
Specificity	This CD68 antibody clone FA-11 does detect surface Macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Formulation	PBS, pH 7.4 Label: Biotin State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Biotin
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD68 antigen
Database Link	Entrez Gene 12514 Mouse UniProt ID P31996
Background	The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.
Synonyms	Gp110, Macrosialin, Macrophage marker
Reference Data

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