malE Mouse Monoclonal Antibody [Clone ID: R29]

Specifications

Product Data
Clone Name	R29
Applications	IP, WB
Recommend Dilution	Western blot. Immunoprecipitation.
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Maltose Binding protein. Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognizes maltose binding protein (MBP). The antibody is useful in recognising proteins expressed with an MBP tag.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P0AEX9
Background	Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
Synonyms	MMBP, Maltodextrin-binding protein, malE-Tag
Reference Data

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