Fast skeletal muscle Troponin T Mouse Monoclonal Antibody [Clone ID: T1/61]

Specifications

Product Data
Clone Name	T1/61
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot. Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections; This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control Tissue: Skeletal muscle.
Reactivity	Chicken, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Troponin T, purified from chicken breast muscle. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises fast muscle Troponin T.
Formulation	PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P12620
Background	The contraction of striated muscle is initiated by the binding of intracellular calcium to the troponin complex located in the thin filaments. The complex consists of the 3 different troponins; troponin C, troponin T, and troponin I. Troponin T is the tropomyosin binding subunit of troponin which confers calcium sensitivity to striated muscle actomyosin ATPase activity. TNNT3 (Troponin T type 3 (skeletal, fast)) is one of 3 identified troponin T proteins which are encoded by different genes.
Synonyms	TnTf, Beta TnTF, TNNT3
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.