TSH Receptor (TSHR) Mouse Monoclonal Antibody [Clone ID: 4C1]

Specifications

Product Data
Clone Name	4C1
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/100-1/1,000 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/1,000-1/2,000 (does not require protein digestion pre-treatment nor antigen retrieval using heat treatment prior to staining of paraffin sections). Histology positive control: Thyroid gland. Immunoprecipitation. Western blotting: 1/1,000. According to customer information, this antibody has been used successfully on a peptide microarray (2). Not Suitable for ELISA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	External domain of Human TSH receptor
Specificity	Recognizes the Human thyroid stimulating hormone (TSH) receptor binding to an extracellular domain. The epitope has been mapped to aa 379-384 (2).
Formulation	PBS containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	thyroid stimulating hormone receptor
Database Link	Entrez Gene 7253 Human UniProt ID P16473
Background	Thyrotropin Receptor, a Glycoprotein Hormone Receptor, plays a central role in controlling thyroid cell metabolism. The TSH receptor can interact with both TSH and autoantibodies against the TSH receptor (thyroid-stimulating immunoglobulins, TSI). Mutations in the receptor are the cause for hyperthyroidism such as gestational hyperthyroidism (hypersensitivity to chorionic gonadotropin) and Grave's disease (autoimmune hyperthyroidism). Multiple isoforms of the thyrotropin receptor are produced by altervative splicing. The thyrotropin receptor has been reported in adipose, adrenal, brain, eye, heart, kidney, skin, thymus, and thyroid. ESTs have been isolated from brain, placenta, and thyroid libraries.
Synonyms	Thyroid-stimulating hormone receptor, TSH-receptor, TSH-R, LGR3
Reference Data

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