Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: B27]

Specifications

Product Data
Clone Name	B27
Applications	FC
Recommend Dilution	Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; Membrane permeabilisation is required for this application.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human IFN gamma.
Specificity	This antibody is specific for interferon gamma (IFN gamma). IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes. Clone B27 is reported to neutralise the antiviral activity of human IFN gamma. We recommends the use of SM1326A for this purpose.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	interferon, gamma
Database Link	Entrez Gene 3458 Human UniProt ID P01579
Background	Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Synonyms	IFN-gamma, gamma IFN
Reference Data

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