HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]

Specifications

Product Data
Clone Name	W6/32
Applications	ELISA, FC, IF, IHC, IP
Recommend Dilution	ELISA. Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 1x106 cells or 100 µl whole blood. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes. Immunohistochemistry on Frozen Sections: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Acetone fixation for frozen sections is recommended. Positive Control: Tonsil Tissue. Does not work on Paraffin Sections.
Reactivity	Bovine, Feline, Human, Monkey, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human tonsil lymphocyte membranes
Specificity	Antibody SM1222P recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (See Ishitani, A. et al. for details). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. Negative Species: Goat, Dog, Guinea Pig, Rabbit, Mouse, Chicken, Amphibia.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Synonyms	MHC class I ABC, HLA Class 2 ABC
Reference Data

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