TPSAB1 Mouse Monoclonal Antibody [Clone ID: AA1]

Specifications

Product Data
Clone Name	AA1
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA. Western Blot. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/10,000. This product requires either protein digestion pre-treatment of paraffin sections e.g. pronase, or HIER. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil.
Reactivity	Canine, Feline, Human, Monkey, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human mast cell tryptase purified from Human lung tissue. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antiobdy recognises Mast Cell Tryptase, both alpha and beta isoforms. This antibody is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tryptase alpha/beta 1
Database Link	Entrez Gene 54271 RatEntrez Gene 7177 Human UniProt ID Q15661
Background	Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
Synonyms	TPSB1, Tryptase I, Mast Cell Tryptase
Reference Data
Protein Families	Druggable Genome, Protease, Secreted Protein

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