CD151 Mouse Monoclonal Antibody [Clone ID: 11G5a]

Specifications

Product Data
Clone Name	11G5a
Applications	ELISA, FC, IHC, IP, WB
Recommend Dilution	ELISA. Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells or 100 µl of whole blood. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/100. This product does not require protein digestion pre-treatment but results are enhanced with with HIER using 0.01M citrate buffer pH6. Recommended Positive Control: Tonsil/spleen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes the CD151 cell surface antigen, also known as PETA-3.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD151 molecule (Raph blood group)
Database Link	Entrez Gene 977 Human UniProt ID P48509
Background	CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen.
Synonyms	PETA-3, PETA3, GP27, Tetraspanin-24, SFA-1, SFA1
Note	It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (ref.1).
Reference Data

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