Integrin beta 4 (ITGB4) Mouse Monoclonal Antibody [Clone ID: 450-9D]

Specifications

Product Data
Clone Name	450-9D
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/25-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunoprecipitation. Western blot. Immunohistochemistry on Frozen Sections. (1/500-1/1000). Does not work on Paraffin Sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified alpha 6/beta 4 Integrin from A431 cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Specificity	This antibody reacts with beta 4 Integrin (CD104). This antibody recognises an extracellular epitope on the CD104 molecule.
Formulation	PBS, pH 7.4, containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit beta 4
Database Link	Entrez Gene 3691 Human UniProt ID P16144
Background	Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities. CD104 is expressed on epithelial cells, Schwann cells and some tumor cell lines.
Synonyms	ITGB4, GP150
Reference Data

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