CD66b (CEACAM8) Mouse Monoclonal Antibody [Clone ID: 80H3]

Specifications

Product Data
Clone Name	80H3
Applications	FC, IHC
Recommend Dilution	Western Blot (See Feuk-Lagerstedt et al). Functional Studies: Removal of Sodium Azide by dialysis is recommended prior to use. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 5x105 cells or 100 µl whole blood. Immunohistochemistry on Frozen Sections: 1/25-1/100. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Please use acetone fixation. Recommended Positive Control: Bone marrow tissue. Does not work on Paraffin Sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human leucocytes from individuals with CML
Specificity	SM1154 recognizes the CGM6 gene product of the carcinoembryonic gene family.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Ascites Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	carcinoembryonic antigen related cell adhesion molecule 8
Database Link	Entrez Gene 1088 Human UniProt ID P31997
Background	CD66b is a 95-100 kD, GPI anchored molecule. CD66b is expressed strongly by mature granulocytes and meta-myelocytes, but only weakly by bone marrow myelocytes. Peripheral blood monocytes and lymphocytes do not express significant amounts of this antigen. The antibody reacts with cells from CML in the chronic phase. The CD67 cluster was re-designated as CD66b at the Vth Leucocyte Typing Workshop in Boston, 1993.
Synonyms	CD67, CGM6, NCA-95
Reference Data

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