LFA3 (CD58) Mouse Monoclonal Antibody [Clone ID: BRIC-5]

Specifications

Product Data
Clone Name	BRIC-5
Applications	FC, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl. Western Blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human erythrocytes.
Specificity	BRIC5 was produced in response to erythrocytes. It reacts by Immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.
Formulation	State: Purified State: Liquid purified IgG containing 0.09% Sodium Azide as preservative.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD58 molecule
Database Link	Entrez Gene 965 Human UniProt ID P19256
Background	CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.
Synonyms	Ag3, LFA-3
Reference Data

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