CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: ERIC-1]

Specifications

Product Data
Clone Name	ERIC-1
Applications	FC, IHC, WB
Recommend Dilution	ELISA: 50 ng/ml. Immunoblotting. Immunohistochemistry on Frozen Sections.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Retinoblastoma tumour.
Specificity	This antibody recognises N-CAM expressed on developing and adult neuroectodermal tissues. Neuroectodermal tumours also stain including Glioma, ependymoma, neuroblastoma, medulloblastoma, retinoblastoma and teratoma. Oat cell carcinoma and Wilms tumour are also highly reactive. ERIC-1 will react on Natural Killer cells and recognises 180, 140 and 120 kD NCAM isoforms.
Formulation	PBS without preservatives State: Azide Free State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Gene Name	neural cell adhesion molecule 1
Database Link	Entrez Gene 4684 Human UniProt ID P13591
Background	NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.
Synonyms	NCAM-1, N-CAM-1, NCAM
Reference Data

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